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[Whole microbial genome shotgun sequencing].

Chun-Qing Luo1, Huan-Ming Yang

  • 1The Human Genome Center, Institute of Genetics and Developmental Biology, CAS, Beijing 100101, China. luochq@genomics.org.cn

Yi Chuan = Hereditas
|August 30, 2005
PubMed
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Whole genome shotgun sequencing is a convenient method for microbial genomes. This article details its library construction, sequencing, assembly, and gap-filling techniques.

Area of Science:

  • Genomics
  • Microbiology

Context:

  • Whole genome sequencing is crucial for understanding microbial life.
  • Two primary strategies exist: clone-by-clone and whole genome shotgun sequencing.

Purpose:

  • To discuss the methodologies and technologies involved in whole microbial genome shotgun sequencing.
  • To provide insights into practical aspects of this sequencing approach.

Summary:

  • This article focuses on whole genome shotgun sequencing, a convenient method for microbial genomes.
  • Key aspects covered include library construction, insert ratios, sequencing depth, assembly algorithms, and gap-filling strategies.
  • Practical examples illustrate the application of these techniques.

Impact:

Related Experiment Videos

  • Facilitates efficient and comprehensive analysis of microbial genomes.
  • Supports advancements in microbiology, biotechnology, and infectious disease research.