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Strain difference of murine bone marrow-derived mast cell functions
Junko Noguchi1, Etsushi Kuroda, Uki Yamashita
1School of Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishiku, Kitakyushu 807-8555, Japan.
Journal of Leukocyte Biology
|August 30, 2005
Summary
Murine mast cell (BMMC) functions differ between BALB/c and C57BL/6 mouse strains, impacting allergic responses. This strain difference in mast cell mediator release influences susceptibility to allergies.
Area of Science:
- Immunology
- Allergy Research
- Cell Biology
Background:
- Mast cells are key players in allergic responses.
- Murine mast cell (BMMC) functions can vary between mouse strains.
- Understanding these differences is crucial for allergy research.
Purpose of the Study:
- To investigate the functional differences between BALB/c and C57BL/6 murine mast cells.
- To determine how these differences contribute to varying allergic susceptibilities.
- To analyze the release of mediators and cytokines by mast cells from different mouse strains.
Main Methods:
- Bone marrow-derived mast cells (BMMC) were cultured from BALB/c and C57BL/6 mice.
- Cells were stimulated with immunoglobulin E (IgE) antibody and antigen.
- Mediator and cytokine release, along with mRNA expression, were quantified.
Main Results:
- C57BL/6 BMMC released more beta-hexosaminidase and histamine.
- BALB/c BMMC showed higher production of prostaglandin D2 (PGD2), IL-6, and monocyte chemoattractant protein-1.
- Hematopoietic PGD synthase (hPGDS) expression was higher in BALB/c BMMC.
Conclusions:
- Significant strain-dependent differences exist in murine mast cell function.
- These functional variations in mast cells may explain differential susceptibility to allergic reactions in mouse strains.
- The study highlights the importance of mouse strain selection in allergy research.