Related Experiment Video
Updated: Jan 24, 2026

Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism
Published on: August 19, 2021
[Study and spectrophotometric determination of trace silver with iodide-Victoria Pure Blue BO system]
1College of Urban Construction, University of Shanghai for Science and Technology, Shanghai 200093, China.
Abstract:
Analytical properties and spectra of naphthyl diphenymethane basic dyes and associated complexes of dye with [AgI2]- was studied. A new method for the spectrophotometric determination of trace amount of silver was developed. The method was based on the Victoria Pure Blue BO reacted with [AgI2]- to form blue associated complex in the buffer medium of H3PO4 -NaH2PO4. The spectrophotometric measurements were directly carried out at 574 nm, the apparent molar absorptivity of associated complex was 7.25 x 10(4) L x mol(-1) x cm(-1). The Beer's law was obeyed in the range of 4-250 microg x L(-1). The recoveries were in the range of 97.0%-98.3%. RSD was 2.7%. The method has been used to determination of trace silver in river water with satisfactory results.
More Related Videos
08:07Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
Published on: September 11, 2018
10:41Enhanced Electron Injection and Exciton Confinement for Pure Blue Quantum-Dot Light-Emitting Diodes by Introducing Partially Oxidized Aluminum Cathode
Published on: May 31, 2018
Related Concept Videos
Determination
Techniques for Isolation of Pure Cultures
Need for Obtaining Pure Cultures
Determining the pH of Salt Solutions
Determining Order of Reaction
Rate-Determining Steps
In a multistep reaction mechanism, one of the elementary steps progresses significantly slower than the others. This slowest step is called the rate-limiting step (or rate-determining step). A reaction cannot proceed faster than its slowest step, and hence, the rate-determining step limits the overall reaction rate.
The concept of rate-determining step can be understood from the analogy of a 4-lane freeway with a short-stretch of traffic-bottleneck caused due to...