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[p53 gene codon 72 polymorphism and susceptibility to keloid]
Yang Zhuo1, Jian-hua Gao, Shen-qiu Luo
1Department of Plastic Surgery, Nanfang Hospital, the First Military Medical University, Guangzhou 510515, China.
Zhonghua Zheng Xing Wai Ke Za Zhi = Zhonghua Zhengxing Waike Zazhi = Chinese Journal of Plastic Surgery
|September 1, 2005
Summary
The p53 Pro/Pro genotype is linked to increased keloid risk in southern Chinese populations. This finding suggests p53 codon 72 genotyping could identify individuals prone to keloid development.
Area of Science:
- Genetics
- Dermatology
- Molecular Biology
Background:
- Keloids are abnormal scars resulting from aberrant wound healing.
- The p53 tumor suppressor gene plays a role in cellular responses to stress and DNA damage.
- Polymorphisms in the p53 gene, such as at codon 72, may influence disease susceptibility.
Purpose of the Study:
- To examine the association between the p53 codon 72 polymorphism and keloid susceptibility.
- To determine if specific p53 genotypes increase the risk of developing keloids in a southern Chinese cohort.
Main Methods:
- Genotyping of the p53 codon 72 polymorphism was performed using polymerase chain reaction-reverse dot blot (PCR-RDB).
- DNA direct sequencing was employed for genotype confirmation.
- Case-control study involving 45 keloid patients and 60 healthy controls.
Main Results:
- The p53 Pro allele frequency was significantly higher in keloid patients compared to controls (P = 0.034).
- Keloid patients exhibited a significantly higher frequency of the p53 Pro/Pro genotype (P = 0.036).
- The p53 Pro/Pro genotype was associated with a 2.4-fold increased risk of developing keloids (Odds Ratio = 2.400, 95% CI: 1.048-5.498).
Conclusions:
- The p53 codon 72 Pro/Pro genotype is a significant risk factor for keloid development in the studied population.
- p53 codon 72 genotyping may serve as a predictive marker for identifying individuals at high risk of keloid formation.