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Updated: Aug 16, 2026
Monitoring Protein Adsorption with Solid-state Nanopores
Published on: December 2, 2011
Plasma protein adsorption to nanofabricated fluorinated polyimide surface
H Kawakami1, Y Okuyama, N Matsumoto
1Department of Applied Chemistry, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan. kawakami-hiroyoshi@c.metro-u.ac.jp
In this article, a series of fluorinated polyimides rubbed at different pressures was prepared, and the plasma protein adsorption on the rubbed polyimide films was evaluated using a micro-bicinchoninic acid protein assay. Interestingly, the amount of plasma protein adsorbed on the polyimide surface strongly depended on the rubbing pressure. The amounts of BSA and Fbg adsorbed on the rubbed polyimide film significantly decreased with an increase in the rubbing pressure. In contrast, the amounts of IgG adsorbed on the rubbed film dramatically increased with an increase in the rubbing pressure. In particular, it is interesting to note that a specific adsorption surface for IgG is formed by the rubbing process. We proposed that the nano-ordered hydrophilic and hydrophobic patternings formed on the top and bottom surfaces on the rubbed fluorinated polyimide film might have a significant influence on the plasma protein adsorption.
In this article, a series of fluorinated polyimides rubbed at different pressures was prepared, and the plasma protein adsorption on the rubbed polyimide films was evaluated using a micro-bicinchoninic acid protein assay. Interestingly, the amount of plasma protein adsorbed on the polyimide surface strongly depended on the rubbing pressure. The amounts of BSA and Fbg adsorbed on the rubbed polyimide film significantly decreased with an increase in the rubbing pressure. In contrast, the amounts of IgG adsorbed on the rubbed film dramatically increased with an increase in the rubbing pressure. In particular, it is interesting to note that a specific adsorption surface for IgG is formed by the rubbing process. We proposed that the nano-ordered hydrophilic and hydrophobic patternings formed on the top and bottom surfaces on the rubbed fluorinated polyimide film might have a significant influence on the plasma protein adsorption.
