Related Experiment Video
Updated: Aug 16, 2026

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface
Published on: July 10, 2014
Microtensile adhesion of sealants to intact enamel
J Perdigão1, J W Fundingsland, S Duarte
1Division of Operative Dentistry, Department of Restorative Sciences, University of Minnesota, Minneapolis, 55455, USA. perdi001@umn.edu
Unlabelled:
Contamination of etched enamel with saliva has been shown to result in sealant failure. Hydrophilic adhesives improve retention of sealants when enamel is contaminated.
Objective:
The objective of the present study was to characterize the adhesion of two sealants to unprepared enamel etched with phosphoric acid or conditioned with the all-in-one, self-etch adhesive Adper Prompt L-Pop.
Methods:
The two proximal enamel surfaces of 16 sound molars were assigned to four conditioning regimens: (1) 35% phosphoric acid for 15 s; (2) Adper Prompt L-Pop, one layer light-cured prior to sealant placement; (3) Adper Prompt L-Pop, two layers light-cured prior to sealant placement; and (4) Adper Prompt L-Pop, one layer co-cured with sealant. One of two sealants, i.e. Clinpro or Delton DDS, was applied, light-cured, followed by a composite build-up to provide support for microtensile bond testing.
Results:
The highest mean bond strengths were obtained when Adper Prompt L-Pop was applied in two layers and light-cured prior to the insertion of Clinpro Sealant. Co-cure of Adper Prompt L-Pop with either sealant resulted in bond strengths which were not significantly different from those of the phosphoric acid control. The use of Adper Prompt L-Pop in one layer cured prior to sealant placement resulted in statistically lower bond strengths than any other application technique regardless of the sealant used. Clinpro Sealant resulted in statistically higher bond strengths than Delton DDS Sealant.
Conclusions:
Application of one layer of Adper Prompt L-Pop co-cured with the sealant resulted in bond strengths similar to those obtained with phosphoric acid etching.
Related Concept Videos
Bonding and Strength of Aggregate
Adherens Junctions
Adherens Junctions are Dynamic
The endothelial cells...