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Updated: Aug 16, 2026

Isolation and Cryopreservation of Highly Viable Human Peripheral Blood Mononuclear Cells From Whole Blood: A Guide for Beginners
10:48

Isolation and Cryopreservation of Highly Viable Human Peripheral Blood Mononuclear Cells From Whole Blood: A Guide for Beginners

Published on: October 25, 2024

[Effect of human plasma on preservation of red blood cells]

Yan Qiu1, Hai-Ping Yang, Yi Zha

  • 1Beijing Red Cross Blood Center, Beijing 100088, China. Qiu444cn@yahoo.com.cn

Zhongguo Shi Yan Xue Ye Xue Za Zhi
|September 1, 2005
PubMed

In order to study whether plasma can affect the structure and function of red blood cells during their storage period, the differences of pH value, concentration of K(+), Na(+), osmotic fragility, plasma hemoglobin, AchE, ATP, 2.3-DPG, P50 in suspended RBC, washed RBC, and RBC with various plasma volume at different storage times were compared. The results showed that plasma helped the blood to keep the RBC at high pH value, low K(+), high Na(+) and maintain RBC-ATP, oxygen carry capacity and deformability, but no effect on maintenance of osmotic fragility, and levels of plasma hemoglobin, AchE, ATP and 2.3-DPG was found in preservated blood. In conclusion, human plasma may be in favour of the preservation of red blood cells.

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