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Updated: Aug 16, 2026
Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage
Published on: February 15, 2020
Influence of UV-irradiation on enzymes in mouse ocular lens: in vitro studies
1Department of Zoology, School of Sciences, Gujarat University, Ahmedabad, India.
Purpose:
In vitro study of the enzymes involved in aerobic, anaerobic and hexose monophosphate shunt in ultraviolet radiation exposed mice lenses.
Method:
Of the selected enzymes, lactic dehydrogenase (LDH) was representative of anaerobic glucose oxidation, succinic dehydrogenase (SDH) of the aerobic oxidation, and Glucose-6-phosphate dehydrogenase (G-6-PDH) of the Hexose Monophosphate (HMP) shunt. Other enzymes studied were ATPase and glutathione reductase (GR).
Results:
Experiments with mice lenses in vitro showed that transparent lens became opaque following UV-irradiation at 360 nm. Opacification of the lens was accompanied by a change in enzyme activities for energy metabolism.
Conclusion:
These changes were progressive in a manner analogous to sequential morphological changes, which would be crucial in maintaining lens transparency.
