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Updated: Aug 16, 2026

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
Published on: September 16, 2025
Statistical methods for comparing comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry
Robert A Shellie1, Werner Welthagen, Jitka Zrostliková
1Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
Abstract:
The potential for the utilization of GC x GC-time-of-flight (TOF) MS for high-resolution metabolomics studies is discussed, with the implementation of some statistical comparisons for biomarker detection. Metabolite profiles from NZO obese mice versus BL/6 control mice are compared and contrasted using a number of chromatogram comparison routines, including direct chromatogram comparisons, chromatogram subtraction and averaging routines, as well as a method for generating relative weighted peak surface difference chromatograms, and a more conventional Student's t-test statistical approach.
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