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Related Experiment Videos

Mouse embryonic stem cells for quality control testing in assisted reproductive technology programs.

John J Kim1, William C Patton, Johannah Corselli

  • 1Department of Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, California 92354, USA. pchann@yahoo.com

The Journal of Reproductive Medicine
|September 1, 2005
PubMed
Summary
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The mouse embryonic stem (ES) cell assay effectively detects embryotoxic materials, showing comparable results to traditional sperm and embryo tests. This assay shows promise for evaluating contact materials and DNA-altering agents in reproductive toxicology.

Area of Science:

  • Reproductive Biology
  • Toxicology
  • Stem Cell Research

Background:

  • Assessing embryotoxicity is crucial for reproductive safety.
  • Traditional bioassays like sperm motility tests and 1-cell mouse embryo bioassays are established methods.
  • Novel assays are needed to improve sensitivity and scope for detecting various embryotoxic agents.

Purpose of the Study:

  • To compare the efficacy of the mouse embryonic stem (ES) cell assay against established sperm motility and 1-cell mouse embryo bioassays.
  • To evaluate the mouse ES cell assay's ability to detect embryotoxic effects from different materials.
  • To determine the sensitivity and specificity of the ES cell assay.

Main Methods:

  • Mouse ES-D3 cells, 1-cell mouse embryos, and sperm were exposed to control and test materials for 1-4 days.

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  • ES cell viability (eosin and Sybr-Gold methods) and apoptosis were assessed.
  • Sperm motility parameters and blastocyst development were measured for comparison.

    • Main Results:

    • The mouse ES cell assay, particularly with Sybr-Gold, demonstrated sensitivity in detecting latex glove-induced toxicity, consistent with standard bioassays.
    • The ES cell assay uniquely identified toxicity from a syringe material, which was missed by other methods.
    • The ES cell assay showed high specificity (100%) and variable sensitivity (33-67%) depending on the viability method used.

    Conclusions:

    • The mouse ES cell assay, especially using Sybr-Gold, is a viable alternative to standard bioassays for detecting embryotoxicity.
    • The ES cell assay is suitable for testing contact materials and DNA-modifying agents.
    • Further research is recommended to optimize the ES cell assay's sensitivity for routine application in assisted reproductive technology.