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[Protective effects of sini decoction on cardiomyocytes in oxidative stress damage induced by peroxide]
Yongmei Nie1, Weikang Wu, Ying Liu
1Department of Pathophysiology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou.
Objective:
To investigate protective effects of Sini decoction on cardiomyocytes from oxidative stress damage induced by peroxide (H2O2).
Methods:
Ventricular cardiomyocytes were prepared from 1-to-2-day-old Sprague-Dawley rats and cultured with DMEM culture media containing 15% neonatal bovine serum. 4 days later, culture media were replaced with neonatal bovine serum-free DMEM with 15%, 1.5% and 0.15% SD rats serum containing Sini decoction, respectively. Viablity percentage of rat cardiomyocytes was detected by flow cytometry using Annexin V-FITC to screen the appropriate SD rat serum concentration containing Sini decoction in DMEM culture media. Cardiomyocytes were exposed to 1 mmol/L H2O2 for 0.5 h, 1 h, 2 h, 4 h and 8 h respectively. MDA and SOD in cardiomyocytes were measured. Apoptosis and necrosis percentage of cardiomyocytes were measured by flow cytometry using Annexin V-FITC as well. Bcl-xL and Bcl-xS mRNA in cardiomyocytes were detected by polymerase chain reaction.
Result:
15% SD rat serum with Sini decoction in DMEM culture midia was the appropriate concentrate for cell growth. MDA and SOD in cardiomyocytes were changed with time elapse of cardiomyocytes exposure to H2O2. Sini decoction could inhibit rat cardiomyocytes from oxidative stress damage induced by H2O2.
Conclusion:
To some extend, Sini decoction inhibit rat cardiomyocytes from oxidative stress damage induced by peroxide through promoting expression of Bcl-xL mRNA and decreasing expression of Bcl-xS mRNA in cardiomyocytes.
