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Updated: Aug 16, 2026
Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation
Published on: September 16, 2018
[Effect of Angelica sinensis polysaccharide on lymphocyte proliferation and cytokine induction]
Tiehong Yang1, Min Jia, Qibing Mei
1Department of Pharmacology, Fourth Military Medical University, Xi'an.
Objective:
To investigate the effects of Angelica sinensis polysaccharide (AP) and its three fractions (AP-1, AP-2 and AP-3) on lymphocyte proliferation induction of IL-2 and IFN-gamma.
Methods:
Effects of AP on proliferation of murine splenocyte, macrophage, mixed lymphocyte reaction were tested by MTT assay. The production of IL-2 and IFN-gamma in supernatant were determined by ELISA.
Results:
At the range of 30 - 300 microg/ml, AP and its three fractions directly stimulated proliferation of murine splenocyte; AP-3 significantly enhanced the proliferation of macrophage and mixed lymphocyte, the treatment of AP-3 augmented the production of IL-2 and IFN-gamma in cell culture supernatant.
Conclusion:
AP could active macrophage and T lymphocyte, also regulate the immune response through upregulating IL-2, IFN-gamma expression.
