Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Toxin detection by a miniaturized in vitro protein expression array.

Qian Mei1, Carl K Fredrickson, Shouguang Jin

  • 1Department of Mechanical and Aerospace Engineering, University of Florida, P.O. Box 116250, Gainesville, Florida 32611, USA.

Analytical Chemistry
|September 1, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Use of liposomal irinotecan with 5-FU and oxaliplatin (NALIRIFOX) in neoadjuvant pancreatic adenocarcinoma: NEO-Nal-IRI trial.

The oncologist·2026
Same author

PA5201 represses type III secretion system by binding to the P<i><sub>exsC</sub></i> promoter in <i>Pseudomonas aeruginosa</i>.

Microbiology spectrum·2026
Same author

Development of a Platform for <i>in-situ</i> Airborne Virus Detection by Interfacing RT-LAMP Assay with a Condensational Growth Collector.

Aerosol science and technology : the journal of the American Association for Aerosol Research·2026
Same author

Glnk Mediates Carbapenem Resistance Through the NtrB/NtrC-OprD Regulatory Pathway in <i>Pseudomonas aeruginosa</i>.

Pathogens (Basel, Switzerland)·2026
Same author

Direct interaction between phosphotransferase LpxT and ArnT modulates polymyxin B resistance in <i>Pseudomonas aeruginosa</i>.

Microbiology spectrum·2026
Same author

A compass in the challenge: the CALLY index as a prognostic biomarker in advanced cholangiocarcinoma treated with chemoimmunotherapy: a retrospective propensity score-matched cohort study.

Frontiers in immunology·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
Same journal

Development of a Tapping-Mode Scanning Probe Electrospray Ionization Platform for High-Sensitivity and Long-Term Stability in Single-Cell Mass Spectrometry Imaging of Tissue.

Analytical chemistry·2026
Same journal

A Solid-State Near-Infrared Fluorescent Probe by a Synergistic Extended Conjugated System for Detecting Cys with Long-Term Imaging in Orthotopic Bladder Cancer.

Analytical chemistry·2026
Same journal

Air-Based Porous Array Dielectric Barrier Discharge Ionization Source for Explosive Trace Detection.

Analytical chemistry·2026
Same journal

PSAQ<sup>+1</sup>: Absolute Protein Quantification Using a <sup>13</sup>C<sub>1</sub>-Labeled Protein Standard, Coisolation of Peptide Pairs and LC-PRM.

Analytical chemistry·2026
Same journal

Heterojunction-Enhanced Interfacial Evanescent-Tunable Fiber Optic Probe for Amplification-free CRISPR/Cas12a-Based Rapid and Ultrasensitive Detection of MPXV.

Analytical chemistry·2026
See all related articles

This study introduces a novel toxin detection method using differential protein expression inhibition. The unique response patterns generated can identify unknown agents and aid in drug screening.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biosensor Technology

Background:

  • Protein synthesis, involving DNA transcription and protein translation, is fundamental to biological systems.
  • Toxins can interfere with protein expression, offering a potential mechanism for detection.
  • Existing detection methods may lack specificity or the ability to identify unknown agents.

Purpose of the Study:

  • To present a novel concept for toxin detection based on differential inhibition of protein synthesis.
  • To demonstrate the feasibility of this concept using an array-based device and specific protein expression systems.
  • To explore the potential application of this technology in high-throughput drug screening.

Main Methods:

  • Simultaneous in vitro protein expression of multiple proteins (GFP, CAT, luciferase) in an array of miniaturized wells.

Related Experiment Videos

  • Application of toxin simulants (tetracycline and cycloheximide) to observe differential inhibition effects.
  • Analysis of response patterns generated by the array device to identify toxins based on their inhibitory signatures.

    • Main Results:

    • Tetracycline differentially inhibited green fluorescent protein (GFP) and chloramphenicol acetyltransferase (CAT) production in an E. coli system.
    • Cycloheximide showed a significant inhibitory effect on luciferase production in a rabbit reticulocyte system, while tetracycline had a negligible effect.
    • A distinct response pattern was achieved in a 3x4 array device, confirming the feasibility of toxin detection via differential inhibition.

    Conclusions:

    • The developed array device and method enable toxin detection and identification based on specific inhibitory response patterns.
    • This approach holds promise for identifying both known and unknown toxic agents.
    • The technology is potentially applicable to high-throughput drug screening due to its mechanism of action.