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Related Experiment Video

Updated: Jul 15, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

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Published on: December 25, 2016

Copper complexes for fluorescence-based NO detection in aqueous solution.

Mi Hee Lim¹, Stephen J Lippard

¹Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Journal of the American Chemical Society

|September 1, 2005

Summary

Two copper(II) complexes with dansylated ligands act as sensitive turn-on fluorescence sensors for nitric oxide (NO). These novel sensors detect NO down to 10 nM by restoring quenched fluorescence.

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Area of Science:

Coordination Chemistry
Analytical Chemistry
Fluorescence Sensing

Background:

Nitric oxide (NO) is a crucial signaling molecule.
Developing sensitive and selective NO sensors is essential for biological and environmental monitoring.
Copper complexes offer versatile platforms for developing novel sensor applications.

Purpose of the Study:

To design and synthesize novel copper(II) complexes with dansylated ligands for NO sensing.
To investigate the fluorescence response of these complexes to nitric oxide.
To determine the detection limit and sensing mechanism of the developed NO sensors.

Main Methods:

Synthesis of two copper(II) complexes incorporating dansylated ligands.
Fluorescence spectroscopy was employed to monitor the response to nitric oxide.
Investigation of the sensing mechanism through spectroscopic analysis and consideration of redox and protonation events.

Main Results:

Both copper(II) complexes exhibited a turn-on fluorescence response upon addition of nitric oxide.
The fluorescence quenching was effectively restored in both organic and aqueous buffered solutions.
A low detection limit of 10 nM for nitric oxide was achieved with these complexes.
The sensing mechanism involves the formation of a diamagnetic Cu(I) species and protonation of the sulfonamide group.

Conclusions:

The developed copper(II) complexes are effective turn-on fluorescence sensors for nitric oxide.
These sensors demonstrate high sensitivity with a detection limit in the nanomolar range.
The unique sensing mechanism provides a basis for designing advanced NO detection probes.