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Soluble glycoprotein V as a quality marker of platelet concentrates stressed by transportation
Kaija Javela1, Juha Eronen, Seppo Sarna
1Finnish Red Cross Blood Service, Kivihaantie 7, FIN-00310 Helsinki, Finland. kaija.javela@bts.redcross.fi
Insights
Soluble glycoprotein V (sGPV) may serve as a quality control marker for platelet concentrates (PCs), correlating with other activation markers. Repeat transportation minimally impacts platelet properties.
Area of Science:
- Transfusion Medicine
- Hematology
- Biochemistry
Background:
- Platelet concentrates (PCs) undergo activation during storage, leading to subtle changes.
- Platelet activation markers include increased P-selectin (CD62P) expression and release of soluble glycoprotein V (sGPV).
- Soluble glycoprotein V (sGPV) is a potential indicator of platelet activation.
Purpose of the Study:
- To evaluate the utility of various assays for quality control of platelet concentrates (PCs).
- To assess the correlation of soluble glycoprotein V (sGPV) with other platelet activation markers and metabolic parameters.
- To determine the impact of transportation on platelet properties.
Main Methods:
- Assessed platelet count, swirling, mean platelet volume, extent of shape change (ESC), hypotonic shock response (HSR), CD62P, CD63, sGPV, and metabolic tests (pH, pO2, pCO2, lactate, glucose).
- Compared assays for buffy coat-derived PCs stored on a flatbed agitator versus those subjected to two overnight transportations.
- Evaluated assay repeatability and correlations between different parameters.
Main Results:
- All tested assays demonstrated good repeatability.
- Significant correlations were observed between sGPV and extent of shape change (ESC), hypotonic shock response (HSR), lactate, glucose, CD62P, and CD63.
- Overnight transportation did not induce significant changes in in vitro platelet properties.
Conclusions:
- Soluble glycoprotein V (sGPV) shows potential as a marker for statistical process control of platelet concentrate (PC) quality, complementing metabolic tests.
- sGPV may aid in evaluating improvements in blood component processing.
- Repeat transportation of PCs has minimal impact on their in vitro properties.
Background:
Despite ongoing improvements in storage conditions for platelet concentrates (PCs) for clinical use, leukoreduced platelets (PLTs) undergo subtle changes that are partly due to PLT activation. As PLTs are activated, the expression of P-selectin (CD62P) increases, and soluble glycoprotein V (sGPV) is released. GPV, part of the GPIbIXV complex, has been suggested as a marker of PLT activation.
Study Design And Methods:
An array of assays, used for quality control of PCs, was performed and the results were compared. The tests included PLT count, swirling, mean PLT volume, extent of shape change (ESC), hypotonic shock response (HSR), CD62P, lysosomal membrane protein (CD63), sGPV, and the metabolic tests (pH, pO(2), pCO(2), lactate, glucose). The performance of the assays was evaluated during the storage period by comparing buffy coat-derived PCs (24 PCs of 4 units) stored on flatbed agitator or stressed twice by overnight transportation.
Results:
The repeatability of all tests was good. ESC and HSR correlated with each other (r = 0.559). Importantly, there were also associations between sGPV and ESC (r = -0.564) and HSR (r = -0.389). The correlations of sGPV with lactate and glucose concentrations and with expression of CD62P and CD63 were also good. No significant changes were induced by two overnight transportations.
Conclusion:
sGPV might be applicable for statistical process control of the quality of PCs, in addition to metabolic tests. It may also be helpful in analyzing potential improvements in blood component processing. Repeat transportation of PCs may cause minimal changes on PLT in vitro properties, if any.
