Related Experiment Video
Updated: Aug 16, 2026

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly
Published on: February 9, 2015
Regulated expression of functional external guide sequences in mammalian cells using a U6 RNA polymerase III promoter
Elizaveta Kovrigina1, Li Yang, Emmanuel Pfund
1Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.
Abstract:
A regulatable promoter has been stably integrated into a human embryonic kidney cell line. The promoter is a pol III mouse promoter and is under the control of ponasterone A, an ecdysone inducer. The promoter controls transcription of an external guide sequence (EGS) targeted against Rpp38, a protein subunit of human RNase P, or of lamin A/C, a gene product located in the nucleus. The amounts of protein of both gene products are severely reduced when the EGSs are made. Several other, but not all, of the protein subunits of RNase P are also inhibited in both mRNA and protein levels when Rpp38 mRNA is targeted.
Related Concept Videos
Reporter Genes
Commonly used reporter...
Regulation of Expression at Multiple Steps
Transcription Initiation
The promoters and enhancers and their accessory proteins allow tight regulation of...
The Eukaryotic Promoter Region
RNA Polymerase II Accessory Proteins
Regulated mRNA Transport

