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Evaluation of Caspase Activation to Assess Innate Immune Cell Death
Published on: January 20, 2023
Programmed cell death 6 (PDCD6) protein interacts with death-associated protein kinase 1 (DAPk1): additive effect on
Jeong Heon Lee1, Seung Bae Rho, Taehoon Chun
1Department of Obstetrics and Gynecology, Chonbuk National University Medical School, Keumam Dong, Dukjin Ku, 561-712, Jeonju, South Korea.
Abstract:
Programmed cell death 6 (PDCD6) protein is a 22 kDa EF-hand type Ca(2+)-binding protein involved in apoptosis. To define the regulating mechanism of PDCD6 activity in the apoptotic pathway, we searched a human ovary cDNA library for a novel PDCD6 binding protein using a yeast two-hybrid system. The selected protein was the human death-associated protein kinase 1 (DAPk1), another protein that functions as a positive mediator of apoptosis. Co-transfection of PDCD6 and DAPk1 cDNA into a tumor cell line accelerated apoptosis via caspase-3 dependent pathway.
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