Implication of genetic variations in congenital obstructive nephropathy

The renin-angiotensin system (RAS) has long been implicated in kidney development, and it has been reported that disruption of angiotensin type 2 receptor (AGTR2) results in a wide range of congenital anomalies of the kidney and urinary tract. We investigated the allele frequencies of the AGTR2 and other RAS genes in Korean patients with ureteropelvic junction obstruction, multicystic dysplastic kidney (MCDK), and unilateral renal agenesis (RA). Fifty-three Korean children were enrolled: 37 boys and 16 girls, 27 with hydronephrosis, 23 with MCDK, and 3 with RA. Among 100 healthy Koreans, the frequencies of A and G alleles at the A-G transition site of intron 1 of the AGTR2 gene were 70% (140/200) and 30% (60/200), respectively. In the patient group, the A allele frequency was 57% (60/106) and the G allele frequency was 43% (46/106), significantly higher than in the general population (P=0.024). There was no significant difference of allele frequency between boys and girls. Angiotensin-converting enzyme insertion/deletion, angiotensinogen M235T, and the angiotensin 2 type 1 receptor A1166C genotype distribution showed no difference from those of the control subjects. These findings indicate that the AGTR2 gene may play a major role in the development of congenital obstructive nephropathy.