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Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
Published on: June 17, 2019
[Study on 9 STR loci polymorphism from Chinese Yao ethnic group(Guangxi)]
Fang Gao1, Shi-hua Bi, Jiang-hua Lai
1National Laboratory of Forensic Sciences, Xian Jiaotong University, Xi'an 710061, China. Gaochina@hotmail.com
Abstract:
Genetic polymorphism of nine STR loci was investigated from a Chinese Yao population based on STR Genescan. Sixty one alleles was determined for 9 loci, such as D3S1358,vWA,FGA,THO1,TPOX,CSF1P0,D5S818,D13S317 and D7S820 with their frequencies 0.0054-0.5924. The average heterozygosity(H) was 0.7357, polymorphism information content(PIC) was 0.6887, the accumulative discrimination power(DP) was 2.02x10(-10) and the probability of paternity exclusion(PPE) was 0.9999. These results suggested that the nine STR loci are very useful for human identification, such as analyzing forensic casework,establishing DNA databases, processing paternity test and studying gene natural resources.
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