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Updated: Aug 16, 2026

Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
Published on: February 22, 2017
Virus attachment and replication are promoted after acquisition of host CD28 and CD152 by HIV-1
Jean-Francois Giguere1, Juliette Diou, Joaquim Madrenas
1Research Center in Infectious Diseases, Laval University Hospital Research Center, and Faculty of Medicine, Laval University, Quebec, Canada.
Abstract:
CD28 is constitutively expressed on CD4(+) cells, but its homologue CD152 is only weakly expressed after cell activation. To determine whether these 2 costimulatory molecules can be inserted into human immunodeficiency virus type 1 (HIV-1), virus was produced in CD28- and CD152-expressing Jurkat-derived cells. Both molecules were efficiently acquired by virions. Virus attachment and infectivity were more affected by CD152 than by CD28. Given that CD28/CD152-CD80/CD86 interactions play a dominant role in antigen presentation, it can thus be proposed that the association between virus-anchored host CD28/CD152 and cell-surface CD80/CD86 on target cells might have consequences for the transmission and pathogenesis of HIV-1.
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