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Coupling on-chip solid-phase extraction to electrospray mass spectrometry through an integrated electrospray tip.
Yanou Yang1, Chen Li, Kelvin H Lee
1School of Applied and Engineering Physics, Cornell University, Ithaca, NY, USA.
Electrophoresis
|September 2, 2005
Summary
This study integrates on-chip solid-phase extraction (SPE) with mass spectrometry (MS) using a novel monolithic column for efficient sample precleaning and preconcentration, demonstrating its utility in analyzing pharmaceutical compounds in urine.
Area of Science:
- Analytical Chemistry
- Microfluidics
- Separation Science
Background:
- Traditional sample preparation methods for mass spectrometry can be time-consuming and labor-intensive.
- Integrating sample precleaning and preconcentration directly onto a microfluidic chip can streamline analytical workflows.
Purpose of the Study:
- To develop and evaluate an integrated on-chip solid-phase extraction (SPE) system coupled with mass spectrometry (MS).
- To demonstrate the capability of the system for sample precleaning and preconcentration using a novel monolithic column.
Main Methods:
- Fabrication of a microchip with integrated monolithic SPE columns using hot embossing and in situ polymerization.
- Interfacing the microchip with an ion trap mass spectrometer via a modified commercial system.
- Monitoring the adsorption and elution of imipramine using mass spectrometry.
Main Results:
- Successful fabrication of microchips with alkylacrylate-based monolithic columns.
- Demonstrated effective adsorption and elution of the pharmaceutical compound imipramine.
- Validated the system's potential for sample cleanup by analyzing spiked urine samples.
Conclusions:
- The developed on-chip SPE-MS system offers an efficient approach for sample precleaning and preconcentration.
- This integrated microfluidic device shows promise for simplifying and enhancing complex sample analyses.