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Updated: Aug 16, 2026
Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
Published on: August 29, 2018
[Dynamic pattern of gene expression in rat hearts responding to transient ischemia/reperfusion detected by cDNA
Can Yuan1, Zhen-Yu Zhao, Ying Liu
1Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410078, China. xianzhongxiao@hotmail.com
Objective:
To analyze the pattern of gene expression programs during rat myocardial ischemia/reperfusion at multiple time points.
Methods:
Rat model of myocardial ischemia/reperfusion was established by repeating the occlusion and relaxation of left coronary artery. cDNA microarray was used to analyze the pattern of gene expression programs in rat myocardium at 1, 3, 6, 12, 24 hours after reperfusion. SOM cluster analysis was used to identify different cluster of genes in which each cluster had similar expression pattern.
Results:
Altogether 75, 779, 205, 155, and 166 genes were differentially expressed at 1, 3, 6, 12, 24 hours after the reperfusion respectively. Clusters analysis identified 12 clusters of genes in which each cluster had similar expression pattern.
Conclusion:
Analysis of gene expression pattern revealed sequential induction of subsets of genes that characterize each response.
