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Updated: Aug 16, 2026

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice
Published on: September 25, 2019
[Effect of interleukin-12 on HBV pan-S gene vaccine]
De-Ming Tan1, Yong-Feng Yang, Shui-Ping Liu
1Institute of Infectious Disease, Xiangya Hospital, Central South University, Changsha 410008, China.
Objective:
To investigate the effect of interleukin-12 (IL-12) on immune responses induced by pcDNA3 which expresses hepatitis B virus pan-S gene.
Methods:
BABL/c mice were immunized with pcDNA3 alone (control group), pcDNA3-PS alone, or co-immunized with pcDNA3-PS and pcDNA3-IL12. Six weeks after the immunization, sero anti-HBS antibody was examined, lymphocyte proliferation test and specific cytokine level were assayed. RESULTS The titer of anti-HBs antibody, the stimulation index of splenocytes, and cytokine level of IL-12 and IFN-gamma from the mice coimmunized with pcDNA3-PS and pcDNA3-IL12 were significantly higher than those of the mice immunized alone with pcDNA3-PS. Antibody level, SI, and cytokine level of the immunized mice were higher than those of the control group.
Conclusion:
IL-12 expression plasmid can act as immunological adjuvants for HBV pan-S gene vaccine.

