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Updated: May 25, 2026
Modeling Chemotherapy Resistant Leukemia In Vitro
Published on: February 9, 2016
[Mechanisms of dibutyl phthalate to purge leukemic cells]
Wu-Qing Wan1, Xiao-Yan Liu, Bing Deng
1Department of Paediatrics, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Objective:
To determine the mechanism of dibutyl phthalate (DBP) to purge leukemic cells by inducing apoptosis.
Methods:
The effects of DBP on the intracellular free Ca2+ concentration ([Ca2+]i) of leukemic cells were measured by Fura-2AM method. The effects of DBP on the protein expression of c-myc and bcl-2 genes of leukemic cells were measured by immunohistochemical assay.
Results:
DBP elicited an intracellular Ca2+ redistribution and a potent extracellular calcium influx in leukemic cells. It also down-regulated the protein expression of c-myc and bcl-2 genes.
Conclusion:
DBP can purge leukemic cells in vitro by increasing [Ca2+]i in cells to initiate apoptosis and down-regulate bcl-2 and c-myc proto-gene expression to promote the cell apoptosis.
