Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple roles for separase auto-cleavage during the G2/M transition.

Maura Papi1, Eli Berdougo, Catherine L Randall

  • 1Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA.

Nature Cell Biology
|September 3, 2005
PubMed
Summary

Separase auto-cleavage is crucial for human cells to enter and progress through mitosis. This process ensures proper spindle assembly and chromosome alignment for efficient cell division.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influence of Menopause Onset on the Progression of Lumbar Degenerative Disease: A Clinical Correlation Study.

Journal of orthopaedic case reports·2026
Same author

Examining spousal and family support as predictors of long-term weight loss and remission of type 2 diabetes following bariatric surgery in Singapore: a retrospective cohort study.

BMJ open·2023
Same author

Bariatric Surgery in Kidney Transplant Candidates and Recipients: Experience at an Asian Center.

Journal of obesity & metabolic syndrome·2022
Same author

Revisional Surgery or Pharmacotherapy for Insufficient Weight Loss and Weight Regain After Primary Bariatric Procedure: a Descriptive Study.

Obesity surgery·2022
Same author

Safety and early efficacy of endoscopic sleeve gastroplasty (ESG) for obesity in a multi-ethnic Asian population in Singapore.

JGH open : an open access journal of gastroenterology and hepatology·2021
Same author

Renal Outcomes 1 Year After Metabolic Bariatric Surgery in a Multi-ethnic Asian Cohort.

Obesity surgery·2021

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Separase, a cysteine protease, initiates anaphase by cleaving cohesin, a key protein complex in chromosome segregation.
  • While human separase undergoes auto-cleavage, its biological role has remained unclear.

Discussion:

  • Preventing separase auto-cleavage in human somatic cells disrupts mitotic entry and progression.
  • This disruption involves the aberrant stabilization of Wee1, a kinase that inhibits mitosis, and is independent of the G2 DNA damage checkpoint.
  • Cells lacking separase auto-cleavage display significant defects in spindle assembly and chromosome alignment during M phase, indicating an early mitotic function for separase.

Key Insights:

  • Separase auto-cleavage is essential for coordinating the G2/M transition and subsequent mitotic events.

Related Experiment Videos

  • The process plays a critical role in ensuring accurate chromosome segregation by regulating mitotic entry and progression.
  • Targeted mutagenesis of the separase locus provides a model to study its multifaceted roles in mitosis.

    • Outlook:

    • Further investigation into the precise mechanisms by which separase auto-cleavage influences Wee1 stability and spindle dynamics.
    • Exploring the therapeutic potential of targeting separase auto-cleavage in cancer, where mitotic errors are common.
    • Elucidating the evolutionary conservation and broader physiological relevance of separase auto-cleavage in different organisms.