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Intranasal administration of ACTH(1-24) stimulates catecholamine secretion
M Yoshida-Hiroi1, Y Tsuchida, G Yoshino
1Division of Respiratory Disease, Department of Internal Medicine, Toho University School of Medicine, Japan.
Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
|September 3, 2005
Summary
Intranasal (IN) ACTH(1-24) effectively stimulates adrenocortical steroids, epinephrine, and norepinephrine. This study confirms IN ACTH administration
Area of Science:
- Endocrinology
- Pharmacology
- Neuroscience
Background:
- Intranasal (IN) adrenocorticotropic hormone (ACTH)(1-24) administration previously shown to stimulate adrenocortical steroid secretion.
- Transmucosal absorption efficiency of ACTH into the adrenal medulla requires further investigation.
Purpose of the Study:
- To determine the transmucosal absorption efficiency of ACTH(1-24) via intranasal administration.
- To assess the impact of intranasal ACTH(1-24) on adrenocortical steroid and catecholamine levels.
Main Methods:
- Seven healthy adult males received 250 microg of ACTH(1-24) via intranasal (IN) or intravenous (IV) routes.
- Serum cortisol, aldosterone, epinephrine, norepinephrine, and dopamine levels were measured at 0, 30, 60, and 120 minutes post-administration.
- Hormone levels were quantified using specific radioimmunoassay (RIA) and high-performance liquid chromatography (HPLC) methods.
Main Results:
- Intranasal ACTH(1-24) administration significantly increased serum cortisol and aldosterone levels, peaking at 30 minutes.
- Both IN and IV ACTH(1-24) administration elevated epinephrine and norepinephrine levels, with epinephrine remaining elevated throughout the sampling period.
- Plasma dopamine levels did not change significantly after IN ACTH(1-24) administration; saline controls showed no significant hormonal changes.
Conclusions:
- Intranasal ACTH(1-24) administration is an effective route for stimulating both adrenocortical steroid secretion and catecholamine release (epinephrine and norepinephrine).
- This study demonstrates the efficacy of transmucosal absorption of ACTH(1-24) through the nasal route for hormonal stimulation.