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De-differentiation-derived mesenchymal stem cells demonstrate selective repression in H19 bioregulatory RNA gene

Robert E Scott1, Sizhi Gao, Chung-Kwan Kim

  • 1Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, Rm. 514 Link Building, 855 Monroe, Memphis, TN 38163, USA. rescott@utmem.edu

Differentiation; Research in Biological Diversity
|September 6, 2005
PubMed

Insights

Cellular de-differentiation confers anticancer activity by repressing H19 RNA expression. This molecular change in de-differentiated cells may explain their resistance to carcinogenesis.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Cellular de-differentiation is linked to anticancer activity and resistance to carcinogenesis.
  • The precise molecular mechanisms underlying this phenomenon remain largely undefined.

Purpose of the Study:

  • To investigate stable molecular alterations associated with cellular de-differentiation.
  • To identify specific genes or pathways involved in the anticancer effects of de-differentiated cells.

Main Methods:

  • DNA microarray analysis of 6,000 genes and 6,000 ESTs comparing control undifferentiated cells with de-differentiated adipocyte-derived clones.
  • Real-time PCR to validate gene expression changes.

Main Results:

  • A consistent, significant difference in gene expression was observed in de-differentiated cells compared to controls.
  • The expression of H19 (a bioregulatory RNA) was repressed more than fourfold in all three de-differentiated cell clones.

Conclusions:

  • Decreased expression of H19 RNA is a stable molecular change associated with cellular de-differentiation.
  • Reduced H19 expression may contribute to the observed anticancer activity and carcinogenesis resistance in de-differentiated cells.

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