Real time PCR assay with fluorescent hybridization probes for genotyping intronic polymorphism in presenilin-1 gene
Miguel Rodriguez-Manotas1, Manuel Amorin-Diaz, Inmaculada Viñals-Bellido
1Clinical Analysis Service, University Hospital Virgen de la Arrixaca, Ctra. Madrid-Cartagena s/n, El Palmar, 30120 Murcia, Spain. MMANOTAS@terra.es
Background:
Identification of all susceptibility loci for Alzheimer's disease has been a major goal in resolving the pathogenesis of this disease.
Methods:
A PCR assay with fluorescently labeled oligonucleotide hybrinization probes with subsequent fluorescent probe melting point analysis was developed.
Results:
Allelic discrimination of intronic polymorphism of presenilin-1 gene and the restriction fragment length polymorphism method yielded identical results, proving its usefulness for genotyping PS1 gene.
Conclusions:
This method provides excellent robustness, speed, and accuracy, and is well suited for determination of the polymorphism in both small and large numbers of samples. This assay could help to overcome the controversy regarding the association between the PS1 s165932 intronic polymorphism and Alzheimer's disease.
