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[Effect of protein kinase C activation on platelet Na+/H+ exchanger]
1Department of Clinical and Laboratory Medicine, Yamanashi Medical College.
Abstract:
Protein kinase C activation in human platelets has a modulatory role in maintaining intracellular pH (pHi), by adjusting pHi at a particular value (pH 7.22). Changes in pHi induced by protein kinase C appeared to depend upon differences between H+ efflux catalyzed by Na+/H+ exchanger and H+ production. The pHi recovery after acid loading was significantly facilitated by protein kinase C activation. Analysis of the rate constant for pHi recovery suggested that the turnover rate or the apparent affinity of the Na+/H+ exchanger for H+ was increased. Protein kinase C also decreased the Km value of the Na+/H+ exchanger for extracellular Na+. It is suggested that the role of protein kinase C in platelet pHi regulation is dual, adjusting the pHi value at a certain set-point, on the one hand, and increasing the rate constant of the Na+/H+, on the other.
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