Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An authentic 3' noncoding region is necessary for efficient poliovirus replication.

David M Brown1, Christopher T Cornell, Genevieve P Tran

  • 1Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, CA 92697, USA.

Journal of Virology
|September 6, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Infographic: Study design for ELARA, an open-label, phase 3b trial of aflibercept 8 mg in previously treated patients with neovascular age-related macular degeneration or diabetic macular edema.

Eye (London, England)·2026
Same author

Efficacy of Continuous Pegcetacoplan Treatment for Subfoveal Geographic Atrophy in Age-Related Macular Degeneration: 36-Month Results from OAKS, DERBY, and GALE Open-Label Extension.

Clinical ophthalmology (Auckland, N.Z.)·2026
Same author

Application of small molecule inhibitors to probe the mechanism underlying nanomaterial pulmonary inflammatory responses <i>in vitro</i>.

Nanotoxicology·2026
Same author

MAGIC: Study Design and Rationale for the Phase 2 Clinical Trial of Faricimab for Non-Proliferative Diabetic Retinopathy.

Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift fur Augenheilkunde·2026
Same author

Varicose Veins and Swelling After Pacemaker Placement.

American family physician·2026
Same author

Athlete maltreatment in sport: Protocol for a scoping review.

PloS one·2025
Same journal

Dysregulation of miRNAs has broad impacts on virus infection in <i>Drosophila</i>.

Journal of virology·2026
Same journal

Identification of GRP78 as a novel host factor that facilitates zoonotic porcine deltacoronavirus internalization and replication via clathrin-mediated endocytosis.

Journal of virology·2026
Same journal

MGF110-2L deletion prevents IFN-I and inflammatory response, resulting in partial attenuation and protection against virulent ASFV.

Journal of virology·2026
Same journal

Periodic genome sequences facilitate packaging in a single-stranded DNA virus.

Journal of virology·2026
Same journal

A novel monoclonal antibody targeting the hemagglutinin-neuraminidase of peste des petits ruminants virus maintains neutralizing activity by blocking viral adsorption and receptor interaction.

Journal of virology·2026
Same journal

ATG9A is an essential host factor for parechovirus RNA replication.

Journal of virology·2026
See all related articles

Investigating poliovirus RNA replication, this study found that a complete 3' noncoding region deletion mutation is infectious. The severe replication defect observed after RNA transfection was partly due to nonviral sequences in the transcripts.

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Picornavirus RNA replication requires synthesis of negative-strand intermediates and accumulation of positive-strand RNA.
  • The 3' noncoding region of viral RNA plays a crucial role in replication initiation.
  • Previous studies suggested compensatory mutations might overcome replication defects.

Purpose of the Study:

  • To identify cis-acting elements essential for initiating negative-strand RNA synthesis in poliovirus.
  • To investigate the replication defect of poliovirus with a deleted 3' noncoding region.
  • To determine the infectious nature of poliovirus RNA lacking its 3' noncoding region.

Main Methods:

  • Deletion of entire 3' noncoding regions from human rhinovirus and poliovirus genomic RNAs.

Related Experiment Videos

  • Transfection of HeLa cells with deletion mutant transcripts and analysis of RNA accumulation.
  • Sequencing of the entire genome of deletion-mutant poliovirus.
  • Transfection analysis of sequence variants to assess compensatory functions.

    • Main Results:

    • Deletion mutant transcripts showed a severe delay in RNA accumulation post-transfection.
    • Subsequent infection revealed only a moderate deficiency in RNA synthesis for deletion-mutant poliovirus.
    • Sequencing identified only two nucleotide changes in the deletion-mutant genome, which did not rescue the replication defect.
    • The severe replication defect was partly attributed to nonviral terminal sequences in in vitro transcripts.

    Conclusions:

    • The severe replication defect in deletion mutants is partially caused by nonviral sequences in the RNA transcripts.
    • Poliovirus RNA with a complete 3' noncoding region deletion is infectious.
    • The 3' noncoding region is critical for efficient RNA replication initiation and accumulation.