Process of destruction of large unilamellar vesicles by a zwitterionic detergent, CHAPS: partition behavior between...

The process of vesicle destruction by zwitterionic detergent, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), was examined to clarify the vesicle-micelle transition mechanism. The physicochemical properties including turbidity, apparent particle size, Cl(-) permeability, electron spin resonance (ESR) spectroscopic parameters, and freeze-fracture electron microscopy were investigated. The concentration of CHAPS was analyzed using HPLC to determine the partition coefficient during the solubilization process. The data obtained revealed that maximum turbidity and apparent particle size were found at the effective ratio (R(e)) of 0.21 and 0.49, respectively. With a further increase in CHAPS concentration, turbidity and particle size abruptly decreased, suggesting the formation of mixed micelles. The partition coefficient changed throughout the solubilization process. In the presence of low concentrations of CHAPS, CHAPS partitioned into vesicles without destruction of membrane bilayers. When the R(e)<0.04, the partition coefficient was independent of the detergent concentration with value of 24 M(-1). At R(e) greater than 0.05, the membrane barrier abruptly decreased. At 0.04