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Related Experiment Videos

A gravimetric simplified method for nucleated marrow cell counting using an injection needle.

Toshiki Saitoh1, Liu Fang, Kiyoshi Matsumoto

  • 1Nippon Institute for Biological Science, Tokyo.

The Journal of Toxicological Sciences
|September 6, 2005
PubMed
Summary

A simplified gravimetric method accurately measures nucleated marrow cell counts (NMC) in rats. This technique offers valuable data for screening and myelotoxicity studies, complementing traditional methods.

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Area of Science:

  • Hematology
  • Toxicology
  • Animal models

Background:

  • Accurate bone marrow cell counting is crucial for assessing hematological health and toxicity.
  • Existing methods may be time-consuming or require specialized expertise.
  • A need exists for a simplified, reliable method for routine screening.

Purpose of the Study:

  • To introduce and validate a simplified gravimetric method for determining nucleated marrow cell counts (NMC) in rats.
  • To assess the feasibility of this method for regular screening and its potential in myelotoxicity studies.

Main Methods:

  • Bone marrow was aspirated from rat femurs and suspended in carbonate buffered saline.
  • Nucleated marrow cell counts (NMC) were determined using an automated multi-blood cell analyzer.

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  • The gravimetric method was applied to Crj:CD(SD)IGS rats aged 4 to 10 weeks.

    • Main Results:

    • The gravimetric method yielded consistent NMC values between left and right femurs in rats.
    • NMC ranged from 2.72 to 1.96 x10^6 counts/mg in male rats and 2.75 to 1.98 x10^6 counts/mg in female rats (4-10 weeks old).
    • The method shows potential for providing additional useful data when combined with myelogram examination.

    Conclusions:

    • The simplified gravimetric method is a viable technique for routine screening of rat bone marrow cell counts.
    • Potential challenges include reduced NMC due to blood contamination and variability from handling.
    • Further data accumulation from myelotoxicity studies is necessary to fully establish the utility of this screening method.