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Updated: Aug 16, 2026

Neutrophil Lifespan Extension with CLON-G and an In Vitro Spontaneous Death Assay
Published on: May 12, 2023
[Non-apoptotic, non-necrotic death of neutrophils induced by EP3 agonist--ONO-AE-248]
Jia-jia Liu1, Zi Zhang, Hao He
1Department of Molecular Biology, Luzhou Medical College, Luzhou 646000, China. jiajialiu08@163.ccom
Aim:
To investigate the effect of EP(3) agonist--ONO-AE-248 on neutrophils.
Methods:
Electron microscopy, MTS assay, RT-PCR and FACS were used to observe the morphology, viability, EP(3) receptor expression and apoptosis of neutrophils treated with ONO-AE-248.
Results:
EP(3) receptor was expressed on neutrophils. ONO-AE-248 rapidly caused a unique form of neutrophil death, showing morphological changes of nucleus, including fusion of the lobules, blebbing and rupture of the nuclear membrane, which were different from typical morphological changes of apoptosis and necrosis. The death was dependent on ATP.
Conclusion:
The death of neutrophils caused by ONO-AE-248 is likely to be one of the forms of "non-apoptotic programmed cell death".
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