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Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
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Published on: December 30, 2014

Phospholipase-C sensitive GPI-anchored proteins of goat sperm: possible role in sperm protection

T E Hutchinson1, A Rastogi, R Prasad

  • 1Reproductive Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Uttaranchal.

Animal Reproduction Science
|September 7, 2005
PubMed

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GPI Anchoring of Proteins in the ER Membrane

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GPI-anchor structure
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Phosphoinositides and PIPs

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Lipids as Anchors

In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
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Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
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