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Published on: December 1, 2020
Synthesis of a biotinylated camptothecin derivative and determination of the binding sequence by T7 phage display
Yoichi Takakusagi1, Keisuke Ohta, Kouji Kuramochi
1Genome and Drug Discovery Research Center, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
Bioorganic & Medicinal Chemistry Letters
|September 7, 2005
Abstract:
A biotinylated derivative of the anti-tumor agent camptothecin (CPT) was synthesized and used in a phage display assay to identify drug-binding sequences. After three rounds of selection using C20-biotinylated CPT (CPT-20-B) as bait, a CPT-20-B-binding sequence, NSSQSARR, was identified.
