Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Anti-CD3 priming generates heterogeneous antigen-specific memory CD4 T cells.

Deepa S Patke1, Mojgan Ahmadzadeh, Adam W Bingaman

  • 1Division of Transplantation, Department of Surgery, University of Maryland School of Medicine, MSTF Building, Room 400, 685 W. Baltimore St., Baltimore, MD 21201, USA.

Clinical Immunology (Orlando, Fla.)
|September 7, 2005
PubMed
Summary

Anti-CD3 activation generates long-lived CD4 T cell memory, mimicking antigen-specific priming. This finding supports anti-CD3

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinct transcription factors control tissue adaptation and effector function in infant and adult memory T cells.

Nature immunology·2026
Same author

Immune development in early life.

Nature immunology·2026
Same author

Age determines NK cell fate and tissue compartmentalization to CMV infection.

bioRxiv : the preprint server for biology·2026
Same author

The human antibacterial factor APOL3 couples lysosomal damage to mitochondrial DNA efflux and type I IFN induction.

Molecular cell·2026
Same author

Preparation of silibinin- dendrimer-stabilized Au nanoparticles for decreasing vancomycin resistance in S. aureus clinical isolates.

International microbiology : the official journal of the Spanish Society for Microbiology·2026
Same author

Tissue signatures of human macrophages during homeostasis and activation.

Journal of immunology (Baltimore, Md. : 1950)·2025

Area of Science:

  • Immunology
  • Cellular Biology
  • Vaccinology

Background:

  • Anti-CD3 antibody activation of T cells is utilized in adoptive immunotherapy for cancer and HIV.
  • The long-term in vivo fate of T cells primed with anti-CD3 remains largely uncharacterized.

Purpose of the Study:

  • To investigate the generation and characteristics of long-lived memory CD4 T cells following anti-CD3 activation.
  • To compare anti-CD3-primed memory T cells with those primed by specific antigen.

Main Methods:

  • Utilized influenza hemagglutinin (HA)-specific TCR-transgenic CD4 T cells in mouse models.
  • Transferred activated T cells into lymphocyte-deficient and intact mouse hosts.
  • Analyzed phenotype, recall function, and proliferative capacity of memory T cells.

Related Experiment Videos

Main Results:

  • Anti-CD3 activation induced a long-lived HA-specific memory CD4 T cell population.
  • This population was phenotypically and functionally similar to HA peptide-primed memory T cells.
  • Anti-CD3 priming resulted in heterogeneous memory subsets distributed in lymphoid and non-lymphoid tissues.

Conclusions:

  • Anti-CD3 activation is effective in generating robust memory T cell responses.
  • This suggests potential applications for anti-CD3 in adoptive immunotherapies and vaccine development.
  • Memory T cell subset heterogeneity and tissue distribution can occur independently of specific antigen or TCR recognition.