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Updated: Aug 16, 2026
Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
Published on: July 30, 2020
[Preparation of cardiomyocyte-targeting liposomes and their targeting ability in vitro]
Yan Chen1, Ying-Jie Deng, Yan-Li Hao
1School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Aim:
To prepare the cardiomyocyte-targeting liposomes and investigate their cardiomyocyte targetability in vitro.
Methods:
Liposomes modified with compound PAC were prepared (PAC-L); The uptake of PAC-L by cardiomyocytes was studied by incubating fluorescence labeled liposomes with cardiomyocytes in vitro and measuring the association of liposomes by a fluorescence spectrophotometer.
Results:
A high affinity of PAC-L to the cardiomyocytes was observed, the amount of cell uptake of PAC-L by cardiomyocytes was higher than that by nonmyocyte (P < 0.001); The amount of cardiomyocyte uptake of PAC-L on the normoxia condition 2 h was 2.9-fold higher than that of plain-L, and the increase was 5.2-fold when hypoxia occured; The form of liposome uptake changed, the amount of cardiomyocyte uptake of Plain-L by internalization was only 11%, while that of PAC-L was 56%.
Conclusion:
It is indicated that PAC-L was a potential drug carrier for targeting to ischemic myocardium.
