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Related Experiment Videos

The basic helix-loop-helix factor Hand 2 regulates autonomic nervous system development.

Yuka Morikawa1, Yan-Shan Dai, Jianming Hao

  • 1Department of Cell and Molecular Biology, Tulane University, New Orleans, Louisiana 70118, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|September 8, 2005
PubMed
Summary

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Hand 2 transcription factor works in parallel with Mash 1 during sympathetic nervous system development. Hand 2 can also activate neuronal programs, suggesting a role in autonomic nervous system development.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Biology

Background:

  • Mammalian autonomic nervous system (ANS) development relies on key transcription factors like Mash 1, Phox 2b, and GATA 3.
  • The precise roles and interactions of these factors in ANS development are still being elucidated.

Purpose of the Study:

  • To investigate the role of the bHLH transcription factor Hand 2 (dHAND) in sympathetic nervous system (SNS) development.
  • To determine the relationship between Hand 2 and Mash 1 during SNS development.
  • To explore the potential of Hand 2 to ectopically activate neuronal programs.

Main Methods:

  • Analysis of Hand 2 and Mash 1 expression during SNS development.
  • Experiments involving ectopic expression of Hand 2 in P19 embryonic carcinoma cells.

Related Experiment Videos

  • Investigation of Hand 2's interaction with histone acetyltransferase p300/CBP.

    • Main Results:

    • Hand 2 is co-expressed with Mash 1 during SNS development, but its expression is independent of Mash 1, suggesting parallel functions.
    • Ectopic Hand 2 expression induces a neuronal phenotype, including SNS lineage characteristics, in P19 cells.
    • Hand 2's function in promoting ANS development is linked to its interaction with p300/CBP, indicating its role within a larger transcriptional complex.

    Conclusions:

    • Hand 2 acts in parallel with other transcription factors like Mash 1 in regulating ANS development.
    • Hand 2 can ectopically activate the neuronal program through cross-regulatory mechanisms, including Mash 1 activation.
    • Hand 2's function is mediated through its interaction with co-activators such as p300/CBP, highlighting its role in a complex transcriptional network for ANS development.