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Published on: May 19, 2023
[Effect of supernatants of bone marrow mononuclear cells on endothelial cell proliferation]
Wen-Liang Liu1, Bang-Liang Ying, Feng-Lei Yu
1Department of Thoracic and Cardiovascular Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China. liuwenliang1859@hotmail.com
Objective:
To define in vitro whether bone marrow mononuclear cells (BM-MNC) secrete vascular endothelial growth factor (VEGF) and to evaluate the effect of BM-MNC on endothelial cell proliferation.
Methods:
Rat BM-MNCs were isolated by centrifugation through a histopaque density gradient. BM-MNCs were cultured 4 weeks in vitro. Conditioned medium was assayed for VEGF and was added to cultured human umbilicus vein endothelial cells (HUVEC) to assess the effect of BM-MNC on endothelial cell (EC) proliferation.
Results:
Levels of VEGF in the conditioned medium increased compared with those in the control culture medium which was not exposed to BM-MNC. The conditioned medium enhanced, in a dose-related manner, HUVEC proliferation.
Conclusion:
BM-MNCs is a valuable source of factors involved in angiogenesis. BM-MNCs secrete potent angiogenic cytokines such as VEGF and induce proliferation of endothelial cells.
