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Characterization of the human MDR1 gene
Miklos Bodor1, Edward J Kelly, Rodney J Ho
1Department of Pharmaceutics, University of Washington, Seattle, Washington 98195-7610, USA.
The AAPS Journal
|September 9, 2005
Summary
The human MDR1 gene, encoding P-glycoprotein (Pgp), was definitively characterized. Its genomic sequence is 209 kb, resolving database discrepancies for this important drug transporter.
Area of Science:
- Genomics
- Molecular Biology
- Pharmacology
Background:
- P-glycoprotein (Pgp) is an ATP-dependent efflux transporter crucial for xenobiotic and toxin protection.
- Pgp is encoded by the human MDR1 gene, located on chromosome 7q21.
- Existing database entries for the MDR1 gene length vary significantly, ranging from 6.3 kb to 210 kb.
Purpose of the Study:
- To accurately characterize the genomic sequence and length of the human MDR1 gene.
- To resolve discrepancies in reported MDR1 gene lengths found in public databases.
Main Methods:
- Genomic DNA was isolated from human cell lines and tissues.
- The MDR1 genomic sequence was analyzed and characterized.
Main Results:
- The complete genomic sequence of the human MDR1 gene was determined to be 209 kb.
- This finding provides a standardized and accurate length for the MDR1 gene.
Conclusions:
- The characterized 209 kb length of the human MDR1 gene provides a definitive reference.
- Accurate MDR1 gene length is essential for understanding Pgp function and genetic variations.