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Updated: Aug 16, 2026

Neutrophil Lifespan Extension with CLON-G and an In Vitro Spontaneous Death Assay
Published on: May 12, 2023
[Apoptosis of neutrophil in peripheral blood in experimental sepsis]
Jian-cheng Xi1, Bao-tong Zhou, Li-xian Jiang
1Department of Orthopaedics, the 309 th Hospital of PLA, Beijing 100091, China.
Objective:
To evaluate the changes in apoptosis of neutrophil in peripheral blood in sepsis in rats.
Methods:
The rat sepsis model was reproduced by cecum ligation and puncture (CLP). One hundred and forty-four rats were randomly divided into normal control group, sham operation group and 2, 6, 12, 24, 48, 72 hours after CLP groups. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) was used to identify neutrophil apoptosis.
Results:
In early period after CLP, neutrophil apoptosis in peripheral blood was limited with a positive rate of less than 5.00%. The positive rate rose to (48.33+/-12.53)% at 48 hours, and it began to lower, approaching the normal level at 72 hours after CLP.
Conclusion:
Death is the main pathway of loss of neutrophils which are produced in the acute phase of sepsis, and apoptosis is the main pathway of loss of neutrophil in the later phase of sepsis.
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