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Related Experiment Videos

Decrease in pH strongly enhances binding of native, proteolyzed, lipolyzed, and oxidized low density lipoprotein

Mia Sneck1, Petri T Kovanen, Katariina Oörni

  • 1Wihuri Research Institute, Helsinki, Finland.

The Journal of Biological Chemistry
|September 9, 2005
PubMed
Summary

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Lowering pH significantly increases low-density lipoprotein (LDL) binding to proteoglycans, promoting its accumulation in arteries. This enhanced binding, especially of modified LDL, contributes to atherosclerosis progression.

Area of Science:

  • Biochemistry
  • Cardiovascular Biology
  • Pathophysiology

Background:

  • Atherogenesis involves low-density lipoprotein (LDL) binding to proteoglycans.
  • Extracellular pH in atherosclerotic lesions decreases during disease progression.

Purpose of the Study:

  • To investigate the impact of decreased extracellular pH on LDL binding to human aortic proteoglycans.
  • To determine how pH affects the binding of native and modified LDL particles.

Main Methods:

  • Microtiter well assays were used to measure LDL-proteoglycan binding.
  • Binding was assessed across a pH range of 5.5 to 7.5.
  • Various LDL modifications (oxidized, proteolyzed, lipolyzed) were tested.

Main Results:

Related Experiment Videos

  • LDL binding to proteoglycans increased significantly as pH decreased.
  • At pH 5.5, binding was 20-37 fold higher than at pH 7.5 for different LDL types.
  • Acidic pH enabled binding of oxidized LDL, which did not bind at neutral pH.
  • Binding was mediated by ionic interactions involving lysine and arginine residues of apolipoprotein B-100.

Conclusions:

  • Decreased extracellular pH in atherosclerotic lesions enhances LDL retention by proteoglycans.
  • This enhanced binding promotes extracellular LDL accumulation, contributing to atherosclerosis progression.