Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Probing lectin and sperm with carbohydrate-modified quantum dots.

Anandakathir Robinson1, Jim-Min Fang, Pi-Tai Chou

  • 1Department of Chemistry, National Taiwan University, Taipei, 106, Taiwan.

Chembiochem : a European Journal of Chemical Biology
|September 9, 2005
PubMed
Summary

Researchers developed beta-N-acetylglucosamine (GlcNAc)-encapsulated quantum dots (QDGLNs) that specifically interact with lectins and sperm. These QDGLNs show distinct binding patterns on sperm, differentiating them from mannose-encapsulated QDs.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TAPINTO: A Novel Algorithm for Tumor-Associated Antigen Prediction Based on Information about Target Overexpression.

Computational and structural biotechnology journal·2026
Same author

A prognostic 19-gene signature and LBP-mediated immune dysregulation define the tumor microenvironment in poor-prognosis KIRC.

International journal of medical sciences·2026
Same author

Synergistic anticancer effects of lipoplex-encapsulated Q7 peptide and doxorubicin against endometrial cancer cells.

Scientific reports·2026
Same author

A SARS-CoV-2 spike-derived adjuvant peptide boosts IL-17/IFN-γ immunity and improves anti-PD-L1 therapy against melanoma.

Molecular medicine (Cambridge, Mass.)·2025
Same author

A Reproducible Sequence-Level Strategy to Enhance Peptide Immunogenicity While Preserving Wild-Type Epitope Recognition.

Antibodies (Basel, Switzerland)·2025
Same author

Chitosan-based Oral DNA vaccine elicits mucosal immunity and reduces Helicobacter pylori colonization in mice.

Vaccine·2025

Area of Science:

  • Bioconjugation chemistry
  • Nanotechnology
  • Glycobiology

Background:

  • Quantum dots offer unique optical properties for biological imaging.
  • Lectins play crucial roles in cell recognition and adhesion.
  • Understanding molecular interactions at the cell surface is vital for diagnostics and therapeutics.

Purpose of the Study:

  • To synthesize and characterize beta-N-acetylglucosamine (GlcNAc)-encapsulated quantum dots (QDGLNs).
  • To investigate the specific multivalent interactions of QDGLNs with lectins and sperm.
  • To compare the binding behavior of QDGLNs with mannose-encapsulated QDs (QDMANs) on sperm.

Main Methods:

  • Quantum dot synthesis and encapsulation with GlcNAc.
  • Fluorimetry for interaction studies.

Related Experiment Videos

  • Transmission electron microscopy (TEM) and dynamic light scattering (DLS) for characterization.
  • Confocal imaging and flow cytometry for cellular analysis.

    • Main Results:

    • QDGLNs demonstrated specific binding to wheat germ agglutinin, leading to fluorescence quenching and aggregation.
    • QDGLNs exhibited site-specific binding to the head of sperm.
    • QDMANs showed a different binding pattern, spreading over the entire sperm body.

    Conclusions:

    • GlcNAc-encapsulated quantum dots are effective tools for studying lectin interactions.
    • QDGLNs and QDMANs can differentiate specific binding sites on sperm.
    • This technology holds potential for sperm-based diagnostics and research.