Related Experiment Video
Updated: Aug 16, 2026
08:42
Establishment of a Co-culture System of Patient-Derived Colorectal Tumor Organoids and Tumor-Infiltrating Lymphocytes (TILs)
Published on: June 27, 2025
Persistence of high CD40 and CD40L expression after restorative proctocolectomy for ulcerative colitis
Lino Polese1, Imerio Angriman, De Franchis Giuseppe
1Clinica Chirurgica I, Policlinico Universitario, via Giustiniani 2, Padova 35128, Italy. linopolese@hotmail.com
World Journal of Gastroenterology
|September 9, 2005
Summary
High CD40 and CD40L expression in the ileal pouch mucosa may contribute to pouchitis after ulcerative colitis surgery. Increased CD40 on blood B cells is linked to extraintestinal issues in these patients.
Area of Science:
- Immunology
- Gastroenterology
Background:
- Ulcerative colitis (UC) patients undergoing restorative proctocolectomy (RPC) can develop pouchitis.
- The role of CD40 and CD40 Ligand (CD40L) in pouchitis pathogenesis is not fully understood.
Purpose of the Study:
- To investigate the expression and role of CD40 and CD40L in the pathogenesis of pouchitis.
Main Methods:
- Immunohistochemistry analyzed CD40 and CD40L in pouch mucosa from 28 RPC patients, 6 UC patients, and 11 controls.
- Flow cytometry assessed CD40 on peripheral blood B lymphocytes and monocytes in 20 pouch patients, 15 UC patients, and 11 controls.
Main Results:
- Ileal pouch mucosa showed significantly higher CD40 and CD40L expression compared to controls, correlating with pouchitis.
- Elevated CD40 on peripheral B lymphocytes was observed in UC and pouch patients versus controls.
- Increased blood B cell CD40 correlated with spondyloarthropathy but not pouchitis.
Conclusions:
- High CD40 expression in ileal pouch mucosa may play a role in pouchitis development post-RPC for UC.
- Elevated CD40 on peripheral blood B lymphocytes is associated with extraintestinal manifestations in pouch patients.
Related Concept Videos
01:20Inflammatory Bowel Disease II: Ulcerative Colitis
Ulcerative colitis is a chronic inflammatory disorder of the colon characterized by continuous mucosal inflammation that typically begins in the rectum and extends proximally in a uniform pattern. Its pathogenesis involves a complex interplay of genetic predisposition, immune dysregulation, and environmental influences. These factors converge to impair the colon’s epithelial defenses and promote an exaggerated inflammatory response against luminal contents.Breakdown of the Mucosal BarrierA...
01:21Inflammatory Bowel Disease V: Surgical Management
Surgical interventions for inflammatory bowel disease (IBD), which includes ulcerative colitis and Crohn's disease, are essential in managing symptoms and addressing complications. The selection of surgical procedures is contingent upon the specific conditions and complications that stem from these illnesses.
Here are some common surgical interventions for IBD:
Here are some common surgical interventions for IBD:
01:20Inflammatory Bowel Disease IV: Clinical Manifestations
Inflammatory bowel disease (IBD) encompasses two major chronic disorders—ulcerative colitis and Crohn’s disease—each characterized by relapsing episodes of gastrointestinal inflammation. Although they share certain clinical features, their patterns of involvement and manifestations differ in ways that aid diagnosis and guide management.Ulcerative ColitisUlcerative colitis is limited to the colon and rectum and involves continuous inflammation of the mucosal layer. The disease course is marked...
01:25Inflammatory Bowel Disease III: Crohn's Disease
Crohn’s disease is a chronic, relapsing form of inflammatory bowel disease characterized by segmental, transmural inflammation that can affect any part of the gastrointestinal tract. Its pathogenesis arises from a combination of genetic susceptibility, environmental exposures, epithelial barrier dysfunction, and immune dysregulation. Together, these factors lead to an exaggerated immune response against components of the gut microbiome.Genetic and Environmental InfluencesMultiple genetic...
01:27Inflammatory Bowel Disease I: Ulcerative Colitis
Introduction
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
01:23Renewal of Intestinal Stem Cells
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...