Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Minireview: aquaporin 2 trafficking.

Giovanna Valenti1, Giuseppe Procino, Grazia Tamma

  • 1Department of General and Environmental Physiology, University of Bari, Italy. g.valenti@biologia.uniba.it

Endocrinology
|September 10, 2005
PubMed
Summary

Aquaporin-2 (AQP2) water channels in the kidney are crucial for regulating water balance. Proper AQP2 trafficking is essential for kidney function and preventing conditions like nephrogenic diabetes insipidus.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanosensing in the Renal Tubule: Ion Channels, Ca 2+ Signaling, and the Integration of Mechanical Cues along the Nephron.

Journal of the American Society of Nephrology : JASN·2026
Same author

Molecular mechanism of p-Coumaric acid in the regulation of AQP2 function through the activation of calcium-sensing receptor signaling.

Scientific reports·2026
Same author

Carrot flour-enriched pasta with <i>in vitro</i> antioxidant activity and capacity of modulating inflammatory pathways in human colon cell model.

Frontiers in nutrition·2026
Same author

Aglianico Grape Pomace Extract Reduces Cardiac Pacemaker Activity by Decreasing Hyperpolarization-Activated Current Density Independently of cAMP Signaling.

Life (Basel, Switzerland)·2026
Same author

Cosmic Kidney Disease: Countermeasures to Attenuate Kidney Damage in Space.

Clinical journal of the American Society of Nephrology : CJASN·2026
Same author

A conserved bioelectrical signature defines subventricular zone-derived human fetal neural stem cells and tracks their differentiation state.

Frontiers in cell and developmental biology·2026

Area of Science:

  • Nephrology
  • Molecular Biology
  • Cell Biology

Background:

  • Aquaporin-2 (AQP2) is key for kidney water transport, regulated by vasopressin.
  • Short-term control involves AQP2 trafficking; long-term control involves AQP2/AQP3 abundance.
  • Defective AQP2 trafficking leads to nephrogenic diabetes insipidus, impairing urine concentration.

Purpose of the Study:

  • To elucidate the mechanisms regulating aquaporin-2 (AQP2) trafficking in the kidney.
  • To understand the role of AQP2 in water homeostasis and kidney diseases.

Main Methods:

  • Investigated AQP2 redistribution via cAMP signaling and V2-receptor activation.
  • Examined the role of protein kinase A-mediated phosphorylation in AQP2 vesicle exocytosis.
  • Assessed the involvement of SNARE proteins and actin cytoskeleton in AQP2 trafficking.

Related Experiment Videos

Main Results:

  • AQP2 is trafficked to the apical membrane via cAMP signaling and V2-receptor activation.
  • Protein kinase A and SNAREs, including VAMP2, are essential for AQP2 vesicle trafficking.
  • Calcium, via calcium-sensing receptors, modulates AQP2 expression and trafficking in vivo.

Conclusions:

  • AQP2 trafficking is a complex process involving signaling cascades, protein interactions, and cytoskeletal elements.
  • Dysregulation of AQP2 trafficking and expression contributes to kidney pathophysiology, including hypercalciuria.
  • Targeting AQP2 pathways offers potential for managing kidney water balance disorders.