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Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
Published on: March 3, 2010
Simple light guide for measuring irradiance in an aqueous oxygen electrode chamber
Jeff Vogtschaller1, Robert Wise
1Department of Botany, University of Wisconsin Madison, 430 Lincoln Dr., WI, USA.
Abstract:
The light-dependent reactions of photosynthesis are often measured with Clark-type oxygen electrodes yet the irradiance level inside aqueous oxygen electrode reaction vessels is seldom reported due to the difficulty of measuring light inside a small volume chamber. We describe a simple light guide terminating in a 90 degrees prism that can be inserted into a reaction vessel. Incoming irradiation is directed to a commercially available quantum sensor positioned at the other end of the light guide. Both materials for and construction of the device are inexpensive.
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