Simple light guide for measuring irradiance in an aqueous oxygen electrode chamber
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Updated: Aug 12, 2026

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
Published on: March 3, 2010
Jeff Vogtschaller1, Robert Wise
1Department of Botany, University of Wisconsin Madison, 430 Lincoln Dr., WI, USA.
Abstract:
The light-dependent reactions of photosynthesis are often measured with Clark-type oxygen electrodes yet the irradiance level inside aqueous oxygen electrode reaction vessels is seldom reported due to the difficulty of measuring light inside a small volume chamber. We describe a simple light guide terminating in a 90 degrees prism that can be inserted into a reaction vessel. Incoming irradiation is directed to a commercially available quantum sensor positioned at the other end of the light guide. Both materials for and construction of the device are inexpensive.
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