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Updated: Aug 10, 2026
HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
Published on: July 20, 2016
Assay of CCNU in tumor by RP-HPLC
Zhiquan Zhao1, Yong Sun, Qiqing Zhang
1National Pharmaceutical Research Center, Lunan Pharmaceutical Corporation, Shandong, China.
Abstract:
This paper reports a RP-HPLC method for the determination of Lomustine (CCNU) in mouse tumor tissue. CCNU was separated and determined on a reverse-phase C18 5 microm column with a mobile phase of H2O-acetonitrile (54:46), detected at 254 nm and no internal standard. The calibration curve was linear (r = 0.9999) within the range of 0.98-15.68 microg/ml for CCNU. The recovery ratio of CCNU was 68.64% (n = 5). The relative standard deviation (RSD) was 0.32%. This method is simple and accurate.
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