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Updated: Aug 2, 2026

Surface Potential Measurement of Bacteria Using Kelvin Probe Force Microscopy
Published on: November 28, 2014
Rapid evaluation of surface sanitation by electrical measurement
Fernando Alvarez1, Susana Sanz, José Juan Rodríguez-Jerez
1Universidad de La Rioja, Departamento de Agricultura y Alimentación, C/Madre de Dios, 51, 26006 Logroño, Spain.
Abstract:
Electrical measurements are easier and faster than traditional methods for evaluating surface sanitation in the food industry. However, the behavior of sublethally damaged bacteria is different in each method. The electrical method is more sensitive to the presence of disinfectant traces in the samples. These issues lead to low correlations between both methods (r2 < 0.7). The use of smaller sample volumes in electrical measurements, together with the addition of a blend of neutralizers to the sample and the subsequent removal by filtration, provide r2 values > 0.9. The developed protocol shows an excellent correlation with traditional methods and facilitates the adaptation of the electrical method to routine monitoring of surface sanitation. It allows for a reduction in holding times and thereby provides the necessary time to make decisions.
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