Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in integrin expression during adipocyte differentiation.

Jun Liu1, Stephanie M DeYoung, Ming Zhang

  • 1Department of Internal Medicine, Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA.

Cell Metabolism
|September 13, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dietary cholesterol activates a Ral-dependent pathway driving LDLR turnover.

Nature·2026
Same author

Time-Restricted Eating Promotes Weight Loss and Favorable Changes in Adipose in Obesity: The TREAD Randomized Control Trial.

Obesity (Silver Spring, Md.)·2026
Same author

A nutrient-responsive AMPK/TBK1 circuit restricts adipocyte catabolism.

JCI insight·2026
Same author

TANK-binding kinase 1 protects against MASH progression via mitochondrial quality control.

Experimental & molecular medicine·2026
Same author

Pacing-induced cardiomyopathy following leadless and transvenous pacemaker implantation: A multicenter retrospective study.

Heart rhythm O2·2026
Same author

Multi-layered transcriptional control of glycogen metabolism coordinates thermogenic remodeling of white adipocytes in male mice.

Nature communications·2025
Same journal

AARS1 promotes tumor progression and immune evasion via ATF6 lactylation-mediated tryptophan metabolism in hepatocellular carcinoma.

Cell metabolism·2026
Same journal

Reactive species as regulators of immune cell metabolism, tolerance, and autoimmunity.

Cell metabolism·2026
Same journal

The interplay between the microbiome and immune cells in metabolic homeostasis and disease.

Cell metabolism·2026
Same journal

The metabolic basis of regulated cell death.

Cell metabolism·2026
Same journal

Gut microbiota-derived lysine phenylacetylation impairs mitochondrial function and is alleviated by SIRT3.

Cell metabolism·2026
Same journal

Methionine-supplemented longevity diet increases growth hormone, GLP-1, and FGF21; reduces frailty; and promotes healthspan.

Cell metabolism·2026
See all related articles

Cyclic adenosine monophosphate (cAMP) regulates adipocyte differentiation by controlling integrin alpha5 and alpha6 expression. Integrin alpha6 is crucial for cell clustering and differentiation, while alpha5 promotes proliferation and inhibits differentiation.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Integrin Signaling

Background:

  • 3T3-L1 preadipocytes require cyclic adenosine monophosphate (cAMP) for maximal differentiation.
  • Integrins are cell surface receptors involved in cell adhesion and signaling.
  • Adipogenesis is a complex process regulated by various signaling pathways and transcription factors.

Purpose of the Study:

  • To investigate the role of integrins alpha5 and alpha6 in cAMP-mediated adipocyte differentiation.
  • To elucidate the downstream signaling pathways regulated by integrins alpha5 and alpha6 during adipogenesis.
  • To understand how integrin-mediated cell adhesion influences preadipocyte proliferation and differentiation.

Main Methods:

  • Microarray analysis to identify cAMP-regulated genes.

Related Experiment Videos

  • Overexpression of integrins alpha5 and alpha6 in 3T3-L1 preadipocytes.
  • Assessment of cell proliferation and differentiation markers.
  • Analysis of GTPase Rac and Rho activity.
  • Cell clustering assays on Matrigel.

    • Main Results:

    • cAMP signaling coordinately regulates integrin alpha5 and alpha6 expression.
    • Integrin alpha5 is downregulated during adipogenesis, while integrin alpha6 is upregulated.
    • Alpha5 overexpression enhances proliferation and inhibits differentiation, involving Rac activation.
    • Alpha6 integrin mediates clustering of growth-arrested preadipocytes, and its perturbation reactivates the cell cycle via Rho.

    Conclusions:

    • Integrin alpha5 promotes preadipocyte proliferation and maintains an undifferentiated state through Rac activation.
    • Integrin alpha6 plays a critical role in cell clustering and terminal differentiation by connecting morphogenesis with signaling.
    • Integrin alpha6 is essential for linking cell adhesion and extracellular matrix interactions to the differentiation process.