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An oligonucleotide barcode for species identification in Trichoderma and Hypocrea
Irina S Druzhinina1, Alexei G Kopchinskiy, Monika Komoń
1Division of Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9/1665, A-1060 Vienna, Austria. druzhini@mail.zserv.tuwien.ac.at
Fungal Genetics and Biology : FG & B
|September 13, 2005
Summary
Accurate identification of Trichoderma species is crucial for research and industry. This study introduces a new oligonucleotide barcode method for rapid and reliable molecular identification of Hypocrea/Trichoderma.
Area of Science:
- Mycology
- Molecular Biology
- Bioinformatics
Background:
- Misidentification of Trichoderma species hinders research comparability.
- Accurate species identification is vital for industrial applications, biocontrol, and ecological studies.
Purpose of the Study:
- To develop a rapid, molecular method for identifying Hypocrea/Trichoderma species.
- To create a user-friendly online tool for species identification.
Main Methods:
- Development of a diagnostic oligonucleotide barcode targeting ITS1 and ITS2 regions of rDNA.
- Creation of a MySQL database storing genus-, clade-, and species-specific oligonucleotide hallmarks.
- Integration of the database into the TrichOKey v. 1.0 web-based identification program.
Main Results:
- The oligonucleotide barcode successfully identified 75 single species, 5 species pairs, and 1 species triplet within Hypocrea/Trichoderma.
- Blind testing on 53 unknown isolates confirmed the barcode's accuracy, aligning with phylogenetic and morphological analyses.
- The TrichOKey v. 1.0 program provides a convenient online platform for molecular identification.
Conclusions:
- The oligonucleotide barcode is a powerful and accurate tool for routine identification of Hypocrea/Trichoderma species.
- This method serves as a valuable complement to traditional identification techniques.
- The TrichOKey v. 1.0 program facilitates accessible and reliable fungal species identification.